使用基于个人计算机的图像分析系统对肌营养不良蛋白基因扩增产物进行定量分析。

Quantitative analysis of dystrophin gene amplification products using a PC-based image analysis system.

作者信息

Minamikawa-Tachino R, Ishii K, Sakuraba H, Suzuki Y, Kaminuma T

机构信息

Computer Center, Tokyo Metropolitan Institute of Medical Science, Japan.

出版信息

Int J Biomed Comput. 1993 Nov;33(3-4):277-86. doi: 10.1016/0020-7101(93)90041-4.

DOI:10.1016/0020-7101(93)90041-4

PMID:8307658

Abstract

A PC-based image analysis system for gel photographs of DNA gel patterns was developed. It was originally designed as a general-purpose, low-cost, yet high-performance system for wide applications in the biomedical area. In this study, we performed analysis of gel images obtained by polymerase chain reaction (PCR) amplification. The system employs a high-resolution CCD camera that can accurately measure grayness of the gel photographs for quantitative analysis of PCR products. The target DNA (exon 52 of the dystrophin gene), which had been found to be deleted in some patients with Duchenne/Becker muscular dystrophy (DMD/BMD), was amplified for the female family members together with the control DNA (exon 60) as a reference. The ratio of the target DNA to the control DNA was determined from PCR products to identify the carrier status of this disease by means of gene dosage. We conclude that 'the PC-based image analysis system' was useful for quantitative deletion analysis of DMD/BMD heterozygotes.

摘要

开发了一种用于DNA凝胶图谱凝胶照片的基于个人计算机的图像分析系统。它最初被设计为一种通用、低成本但高性能的系统，可广泛应用于生物医学领域。在本研究中，我们对通过聚合酶链反应（PCR）扩增获得的凝胶图像进行了分析。该系统采用高分辨率CCD相机，能够准确测量凝胶照片的灰度，以便对PCR产物进行定量分析。对一些杜氏/贝克型肌营养不良症（DMD/BMD）患者中发现缺失的目标DNA（抗肌萎缩蛋白基因外显子52），与作为对照的对照DNA（外显子60）一起对女性家庭成员进行扩增。从PCR产物中确定目标DNA与对照DNA的比例，以通过基因剂量鉴定该疾病的携带者状态。我们得出结论，“基于个人计算机的图像分析系统”可用于DMD/BMD杂合子的定量缺失分析。