Christiansen F T, Tay G, Smith L K, Witt C S, Petersdorf E W, Bradley B, Dawkins R L
Department of Clinical Immunology, Royal Perth Hospital, Western Australia.
Hum Immunol. 1993 Sep;38(1):42-51. doi: 10.1016/0198-8859(93)90518-6.
While the results of unrelated bone marrow transplantation are continually improving, a number of important issues remain: what are the histocompatibility requirements, what genes are involved, what mismatches are acceptable, and what are the best methods for determining donor-recipient match? In this study of material provided through the 4AOHW and the US NMDP, the match between 53 donor-recipient pairs was determined using several different markers within the MHC. The data showed that many apparently well-matched pairs have many mismatches, including mismatches for non-HLA genes (i.e., non-class-I or non-class-II) within the MHC. New methods matching for blocks of DNA around HLA-B and around HLA-DR/DQ are available that are sensitive and identify additional mismatches that are not apparent using conventional typing methods. The 4AOHW cells provide a valuable resource for the comparison and assessment of new matching techniques.
虽然非亲缘骨髓移植的结果在不断改善，但仍存在一些重要问题：组织相容性要求是什么？涉及哪些基因？哪些错配是可接受的？确定供体-受体匹配的最佳方法是什么？在这项通过4AOHW和美国国家骨髓捐赠计划（US NMDP）提供的材料研究中，使用MHC内的几种不同标记确定了53对供体-受体之间的匹配情况。数据表明，许多表面上匹配良好的配对存在许多错配，包括MHC内非HLA基因（即非I类或非II类）的错配。围绕HLA - B以及围绕HLA - DR/DQ的DNA片段匹配的新方法已经出现，这些方法很灵敏，能够识别出使用传统分型方法无法发现的其他错配。4AOHW细胞为新匹配技术的比较和评估提供了宝贵资源。
