Thomsen M, Witt C S, Degli-Esposti M A, Christiansen F T
INSERM U 100, Toulouse, France.
Hum Immunol. 1993 Sep;38(1):52-6. doi: 10.1016/0198-8859(93)90519-7.
Alloreactivity of human NK cells was tested on a subset of the 4AOHW cell panel. A total of 37 cells were typed with NK clones reactive with the NK-1, 2, 3, and 5 allospecificities. No cell was susceptible to lysis by both by anti-NK-1 and anti-NK-2 clones in accordance with the notion of a biallelic system, where the susceptibility toward lysis is a recessive trait. HLA homozygous cells were lysed either by the anti-NK-1 or anti-NK-2 clones while HLA heterozygous cells in some cases were not lysed by either clone. Negativity for the NK-1 specificity corresponded to the presence of asparagine and lysine at positions 77 and 80, respectively, in the second exon of HLA-C (alleles Cw2, 4, 5, and 6), while negativity for the NK-2 group corresponded to the presence of serine and asparagine, respectively, at these two positions (alleles Cw1, 3, 7, and 8). Too few cells were typed with clones reactive with the NK-3 and NK-5 specificities to enable an analysis of correlation between these specificities and HLA alleles.
在4AOHW细胞面板的一个子集上测试了人类自然杀伤细胞（NK细胞）的同种异体反应性。用对NK-1、2、3和5同种特异性有反应的NK克隆对总共37个细胞进行了分型。根据双等位基因系统的概念，没有细胞对抗NK-1和抗NK-2克隆的裂解都敏感，在该系统中，对裂解的敏感性是隐性性状。HLA纯合细胞被抗NK-1或抗NK-2克隆裂解，而在某些情况下，HLA杂合细胞不被任何一个克隆裂解。NK-1特异性的阴性对应于HLA-C第二外显子第77和80位分别存在天冬酰胺和赖氨酸（等位基因Cw2、4、5和6），而NK-2组的阴性对应于这两个位置分别存在丝氨酸和天冬酰胺（等位基因Cw1、3、7和8）。用对NK-3和NK-5特异性有反应的克隆分型的细胞太少，无法分析这些特异性与HLA等位基因之间的相关性。
