Talmadge R J, Roy R R
Department of Physiological Science, University of California, Los Angeles 90024-1527.
J Appl Physiol (1985). 1993 Nov;75(5):2337-40. doi: 10.1152/jappl.1993.75.5.2337.
A new technique for the sodium dodecyl sulfate-polyacrylamide gel electrophoretic separation of rat skeletal muscle myosin heavy-chain (MHC) isoforms is presented. This technique allows for the separation of the four identified MHC isoforms known to be present in adult rat skeletal muscle. These types of MHC are commonly called I, IIa, IIx or IId, and IIb. The procedure can be performed using minigel electrophoresis systems and does not involve preparation of gradient-separating gels or the use of special cooling devices. The procedure accommodates both silver and Coomasie Blue staining. Thus the procedure is simple to perform and highly repeatable, providing high-resolution separation of MHC protein isoforms. The percent composition of the four adult MHCs in rat soleus, medial gastrocnemius, diaphragm, and levator ani muscles by use of this procedure and Coomasie Blue staining is similar to that previously reported. This new technique provides a novel and easy-to-perform method for the separation of rat skeletal muscle MHC isoforms.
本文介绍了一种用于大鼠骨骼肌肌球蛋白重链（MHC）亚型十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的新技术。该技术可分离出已知存在于成年大鼠骨骼肌中的四种已鉴定的MHC亚型。这些类型的MHC通常称为I、IIa、IIx或IId以及IIb。该程序可使用微型凝胶电泳系统进行，无需制备梯度分离凝胶或使用特殊冷却装置。该程序适用于银染和考马斯亮蓝染色。因此，该程序操作简单且高度可重复，可提供MHC蛋白亚型的高分辨率分离。使用该程序和考马斯亮蓝染色法测定大鼠比目鱼肌、内侧腓肠肌、膈肌和提肛肌中四种成年MHC的百分比组成与先前报道的相似。这项新技术为大鼠骨骼肌MHC亚型的分离提供了一种新颖且易于操作的方法。
