Antisense RNA inactivation of gp138 gene expression results in repression of sexual cell fusion in Dictyostelium discoideum.
作者信息
Fang H, Aiba K, Higa M, Urushihara H, Yanagisawa K
机构信息
Institute of Biological Sciences, University of Tsukuba, Japan.
出版信息
J Cell Sci. 1993 Nov;106 ( Pt 3):785-8. doi: 10.1242/jcs.106.3.785.
PMID:8308061
Abstract
A glycoprotein, gp138, is implicated in the sexual cell fusion of Dictyostelium discoideum. We previously cloned and sequenced the two genes encoding the gp138 protein, GP138A and GP138B (Fang et al. (1993) Dev. Biol. 156, 201-208). Here, we have constructed a vector producing antisense RNA for the gp138 genes and have transformed the vector into Dictyostelium cells. The transformed cells showed a reduction in the amounts of gp138 mRNA and protein and their sexual cell fusion activity was considerably repressed.
摘要
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引用本文的文献
1
Targeted disruption of genes for gp138, a cell-fusion-related protein in Dictyostelium discoideum, revealed the existence of a third gene.对盘基网柄菌中一种与细胞融合相关的蛋白gp138的基因进行靶向破坏，揭示了第三个基因的存在。
Dev Growth Differ. 1996 Jun;38(3):271-279. doi: 10.1046/j.1440-169X.1996.t01-2-00006.x.
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