Tomomasa H, Waguri S, Umeda T, Koiso K, Kominami E, Uchiyama Y
Department of Urology, Teikyo University School of Medicine, Tokyo, Japan.
J Histochem Cytochem. 1994 Mar;42(3):417-25. doi: 10.1177/42.3.8308258.
To examine the precise localization of lysosomal cysteine proteinases, cathepsins B, H, and L in rat epididymal epithelial cells, immunohistochemistry and enzyme assay were applied to the epididymal tissue. Granular immunodeposits for cathepsins B and H were detected in epididymal epithelial cells, whereas faint or no immunoreactivity for cathepsin L was found. Moreover, immunoreactivity for cathepsin B appeared mainly in principal cells and was more intense in the head of the epididymis than in the tail, whereas that for cathepsin H appeared in both principal and clear cells and was more intense in the tail than the head. By enzyme assay, activities of cathepsins B and H showed a similar distribution to that of the immunoreactivity. The cathepsin L-specific activity was distributed evenly in each part of the epididymis and was also detected in epididymal fluids obtained from the body and tail parts. By immunoblotting, proforms of cathepsins B, H, and L were present in the seminal fluid. The results suggest that cathepsins B and H are involved in the intracellular degradation system of epididymal epithelial cells, and proforms of cathepsins B, H, and L may be secreted into the epididymal duct lumen.
为了研究溶酶体半胱氨酸蛋白酶组织蛋白酶B、H和L在大鼠附睾上皮细胞中的精确定位，对附睾组织进行了免疫组化和酶活性检测。在附睾上皮细胞中检测到了组织蛋白酶B和H的颗粒状免疫沉积物，而组织蛋白酶L的免疫反应较弱或未检测到。此外，组织蛋白酶B的免疫反应主要出现在主细胞中，在附睾头部比尾部更强烈，而组织蛋白酶H的免疫反应出现在主细胞和透明细胞中，在尾部比头部更强烈。通过酶活性检测，组织蛋白酶B和H的活性分布与免疫反应性相似。组织蛋白酶L的特异性活性在附睾各部分均匀分布，在取自附睾体部和尾部的附睾液中也能检测到。通过免疫印迹法，在精液中存在组织蛋白酶B、H和L的前体形式。结果表明，组织蛋白酶B和H参与了附睾上皮细胞的细胞内降解系统，并且组织蛋白酶B、H和L的前体形式可能分泌到附睾管腔中。
