Chikilian M L, Bradley T J, Nayar J K, Knight J W
Department of Ecology and Evolutionary Biology, University of California, Irvine 92717.
J Parasitol. 1994 Feb;80(1):133-40.
Ultrastructural aspects of extracellular humoral encapsulation of microfilariae of Brugia malayi in the hemocoel of Anopheles quadrimaculatus were compared with those of intracellular encapsulation of first-stage larvae (L1) of the same parasite species, in the thoracic muscle cells of the same species of mosquito. The results showed that extracellular humoral encapsulation of microfilarial sheaths, and sheathed and exsheathed microfilariae, in the hemocoel of mosquitoes occurs around the parasite within the first 6 hr postingestion, apparently without initial participation of hemocytes. Hemocytes and their remnants were observed near the parasite during the first 6 hr postingestion. Within the next 24 hr, hemocytes attach to the initial humoral capsule. By contrast, intracellular encapsulation of L1S is initiated by the accumulation of a dense cytoplasmic layer derived from the infected thoracic muscle cell. Melanin deposits accumulate in this layer adjacent to the parasite cuticle, again without visible participation of hemocytes.
将四斑按蚊血腔中马来布鲁线虫微丝蚴的细胞外体液包囊的超微结构方面，与同一寄生虫物种的第一期幼虫（L1）在同种蚊子胸肌细胞中的细胞内包囊的超微结构方面进行了比较。结果表明，蚊子血腔中微丝蚴鞘、带鞘和脱鞘微丝蚴的细胞外体液包囊在摄入后最初6小时内在寄生虫周围发生，显然最初没有血细胞参与。在摄入后最初6小时内，在寄生虫附近观察到血细胞及其残余物。在接下来的24小时内，血细胞附着于最初的体液包囊。相比之下，L1S的细胞内包囊由源自受感染胸肌细胞的致密细胞质层的积累引发。黑色素沉积物在该层中紧邻寄生虫角质层积累，同样没有血细胞的明显参与。
