来自嗜热栖热梭菌的木聚糖酶B的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium.
作者信息
Nishimoto Mamoru, Fushinobu Shinya, Miyanaga Akimasa, Wakagi Takayoshi, Shoun Hirofumi, Sakka Kazuo, Ohmiya Kunio, Nirasawa Satoru, Kitaoka Motomitsu, Hayashi Kiyoshi
机构信息
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
出版信息
Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. doi: 10.1107/S090744490302715X. Epub 2004 Jan 23.
Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.
已通过坐滴气相扩散法，使用4mg/ml纯化酶、10.3%（w/v）聚乙二醇1500、8.6%（v/v）甘油和0.34M非离子型磺基甜菜碱195制备并结晶了来自嗜热栖热菌的重组成熟木聚糖酶B。在293K孵育十周后生长出合适的晶体。该晶体属于空间群P2(1)2(1)2(1)，晶胞参数a = 64.76，b = 96.60，c = 138.44 Å。已收集到分辨率为1.80 Å的X射线衍射数据。
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