Panocka I, Ciccocioppo R, Polidori C, Massi M
Department of Behavioral Physiology, Polish Academy of Sciences, Mrokow.
Pharmacol Biochem Behav. 1993 Dec;46(4):857-62. doi: 10.1016/0091-3057(93)90213-d.
The present study evaluated the effect of central injections of the 5-HT2/1C receptor antagonist, ritanserin, on ethanol intake in rats with developed preference for 3% ethanol. Intracerebroventricular (ICV) injections of ritanserin (10 micrograms/rat/day for 10 days) decreased ethanol preference, while subcutaneous (SC) treatment with the same dose was ineffective. Ritanserin ICV, 1 microgram/rat/day, did not reduce alcohol preference. Bilateral injections of ritanserin into the nucleus accumbens (NAC; 0.5 microgram/site/day for 10 days) produced a prompt and very pronounced suppression of ethanol preference, without affecting total fluid intake. Bilateral injections of ritanserin (0.5 microgram/site/day for 10 days) into the ventral tegmental area (VTA) or into the medial prefrontal cortex (MPC) evoked only slight and variable reduction of ethanol preference. Injections of ritanserin, 5 micrograms/site/day, into the VTA gave a nonselective suppression of the ingestive behavior. The present results provide evidence for a central site of action for the effect of ritanserin on ethanol intake and suggest that the NAC might be a highly sensitive site for its action. Since the NAC is a major target of the mesolimbic dopaminergic system, they also suggest that the effect of ritanserin might be due to interference with this system.
本研究评估了向已对3%乙醇产生偏好的大鼠脑内注射5-HT2/1C受体拮抗剂利坦色林对乙醇摄入量的影响。脑室内（ICV）注射利坦色林（10微克/大鼠/天，共10天）降低了乙醇偏好，而相同剂量的皮下（SC）给药则无效。脑室内注射1微克/大鼠/天的利坦色林并未降低酒精偏好。向伏隔核（NAC）双侧注射利坦色林（0.5微克/位点/天，共10天）迅速且显著地抑制了乙醇偏好，而不影响总液体摄入量。向腹侧被盖区（VTA）或内侧前额叶皮质（MPC）双侧注射利坦色林（0.5微克/位点/天，共10天）仅引起乙醇偏好轻微且不定的降低。向VTA注射5微克/位点/天的利坦色林对摄食行为产生非选择性抑制。本研究结果为利坦色林对乙醇摄入的作用提供了中枢作用位点的证据，并表明NAC可能是其作用的高敏位点。由于NAC是中脑边缘多巴胺能系统的主要靶点，这些结果还表明利坦色林的作用可能是由于干扰了该系统。
