Kravtsov O V, Kirsenko O V
Ukr Biokhim Zh. 1975 Jan-Feb;47(1):44-8.
A comparative study was carried out of some properties of "soluble" Na+, K+-ATPase obtained from different subcellular membrane brain structures by means of non-ionic detergents of triton X-100 and digitonin. It is established that temperature and pH-optima of "soluble" Na+, K+-ATPase are close to these optima of the initial membrane preparations. A certain difference is observed in the dynamics of temperature and pH-dependence of Na+, K+-ATPase activity in the extracts from different subcellular structures. The stability of the preparations in storage was investigated. A conclusion is made that more stable enzyme extracts may be obtained by means of digitonin.
采用曲拉通X - 100和洋地黄皂苷等非离子型去污剂,对从不同亚细胞膜脑结构中获得的“可溶性”钠钾ATP酶的某些特性进行了比较研究。结果表明,“可溶性”钠钾ATP酶的温度和pH最佳值与初始膜制剂的这些最佳值相近。在不同亚细胞结构提取物中,钠钾ATP酶活性的温度和pH依赖性动力学存在一定差异。研究了制剂在储存中的稳定性。得出的结论是,使用洋地黄皂苷可以获得更稳定的酶提取物。