通过聚合酶链反应-序列特异性寡核苷酸杂交（PCR-SSO）检测HLA-DPB1基因座的已知和新等位基因

Detection of established and novel alleles of the HLA-DPB1 locus by PCR-SSO.

作者信息

Pickl W F, Faé I, Fischer G F

机构信息

Clinical Department for Blood Group Serology, University of Vienna, Austria.

出版信息

Vox Sang. 1993;65(4):316-9. doi: 10.1111/j.1423-0410.1993.tb02173.x.

DOI:10.1111/j.1423-0410.1993.tb02173.x

PMID:8310684

Abstract

The polymorphism at the HLA-DPB1 locus was investigated in 490 Austrian individuals. Exon 2 sequences of the HLA-DPB1 genes were amplified by polymerase chain reaction and probed with a panel of sequence-specific oligonucleotide probes. HLA-DPB1 genotypes were deduced from the hybridization pattern of the probes. HLA-DPB1 allele frequencies and Hardy-Weinberg equilibrium were calculated. Linkage disequilibria between HLA-DPB1 and HLA-DQA1 alleles were determined. Four novel hybridization patterns indicating new alleles were found.

摘要

对490名奥地利个体的HLA - DPB1基因座多态性进行了研究。通过聚合酶链反应扩增HLA - DPB1基因的第2外显子序列，并用一组序列特异性寡核苷酸探针进行检测。根据探针的杂交模式推断HLA - DPB1基因型。计算HLA - DPB1等位基因频率和哈迪-温伯格平衡。确定HLA - DPB1和HLA - DQA1等位基因之间的连锁不平衡。发现了四种表明新等位基因的新型杂交模式。