Hinshaw J E
Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037.
Biochem Pharmacol. 1994 Jan 13;47(1):15-20. doi: 10.1016/0006-2952(94)90432-4.
Recent structural analyses of the nuclear pore complex (NPC) have described in some detail the numerous sub-domains which make up this supramolecular assembly. Three dimensional image analysis of detergent-extracted NPCs reveals that the NPC framework is made up of spoke units, each containing four major domains, arranged with 822 symmetry. As shown by freeze-drying/metal shadowing techniques, attached to this framework are several peripheral components including particles and fibers on the cytoplasmic face and a cage-like structure on the nucleoplasmic face. While it is known that traffic between cytoplasm and nucleus occurs exclusively through the nuclear pore, the specifics of NPC involvement in such traffic remain unknown at present. Proteins destined for the nucleus contain nuclear localization sequences (NLSs). Several proteins have been identified which bind to these NLSs and may act to direct these proteins to the NPC, either releasing them prior to or remaining attached during translocation through the NPC. These NLS-binding proteins have been localized to the cytoplasm, nucleus, nuclear envelope and nucleolus, suggesting some of these proteins transverse the NPC bound or unbound to NLS-containing proteins.
近期对核孔复合体（NPC）的结构分析已经较为详细地描述了构成这种超分子组装体的众多亚结构域。对用去污剂提取的NPC进行的三维图像分析表明，NPC框架由辐条单元组成，每个辐条单元包含四个主要结构域，呈822对称排列。如冷冻干燥/金属阴影技术所示，附着在这个框架上的有几个外周成分，包括细胞质面上的颗粒和纤维以及核质面上的笼状结构。虽然已知细胞质和细胞核之间的物质运输仅通过核孔进行，但目前NPC在这种运输过程中的具体作用仍不清楚。 destined for the nucleus contain nuclear localization sequences (NLSs). Several proteins have been identified which bind to these NLSs and may act to direct these proteins to the NPC, either releasing them prior to or remaining attached during translocation through the NPC. These NLS-binding proteins have been localized to the cytoplasm, nucleus, nuclear envelope and nucleolus, suggesting some of these proteins transverse the NPC bound or unbound to NLS-containing proteins. （这段英文原文似乎有部分重复且表述不太准确，大致意思是：运往细胞核的蛋白质含有核定位序列（NLSs）。已经鉴定出几种与这些NLSs结合的蛋白质，它们可能将这些蛋白质导向NPC，要么在通过NPC转运之前释放它们，要么在转运过程中保持附着。这些与NLS结合的蛋白质已定位在细胞质、细胞核、核膜和核仁中，这表明其中一些蛋白质在与含NLS的蛋白质结合或未结合的情况下穿过NPC。） 前往细胞核的蛋白质含有核定位序列（NLS）。已经鉴定出几种与这些NLS结合的蛋白质，这些蛋白质可能会将这些蛋白质导向核孔复合体，要么在通过核孔复合体转运之前释放它们，要么在转运过程中保持附着。这些与NLS结合的蛋白质已定位在细胞质、细胞核、核膜和核仁中，这表明其中一些蛋白质在与含NLS的蛋白质结合或未结合的情况下穿过核孔复合体。
