Hermans E, Octave J N, Maloteaux J M
Laboratoire de Neurochimie, Université Catholique de Louvain, Brussels, Belgium.
Biochem Pharmacol. 1994 Jan 13;47(1):89-91. doi: 10.1016/0006-2952(94)90440-5.
After association with intact Chinese hamster ovary (CHO) cells expressing the rat neurotensin receptor, tritiated neurotensin was rapidly internalized. Internalization was maximal after 30 min and accounted for about 90% of the total associated ligand. Neurotensin internalization was not observed at 0-4 degrees and was inhibited by an excess of unlabelled neurotensin or by the neurotensin non peptide antagonist, SR 48692. Moreover, the incubation of intact cells for 30 min with 10 nM neurotensin resulted in a significant decrease in the number of the cell surface neurotensin receptors. These results indicate that the endocytosis of membrane bound neurotensin in transfected CHO cells resulted from the internalization of the ligand-receptor complex inside the cell, through an agonist-induced process.
与表达大鼠神经降压素受体的完整中国仓鼠卵巢（CHO）细胞结合后，氚标记的神经降压素迅速内化。内化在30分钟后达到最大值，约占总结合配体的90%。在0-4摄氏度时未观察到神经降压素的内化，且过量的未标记神经降压素或神经降压素非肽拮抗剂SR 48692可抑制其内化。此外，用10 nM神经降压素将完整细胞孵育30分钟导致细胞表面神经降压素受体数量显著减少。这些结果表明，转染的CHO细胞中膜结合神经降压素的内吞作用是由配体-受体复合物在细胞内通过激动剂诱导的过程内化所致。
