Thomas S, Brake B, Luzio J P, Stanley K, Banting G
Department of Biochemistry, University of Bristol, UK.
Biochim Biophys Acta. 1994 Jan 13;1220(2):219-22. doi: 10.1016/0167-4889(94)90139-2.
Immunoscreening a rat liver cDNA expression library has led to the isolation of a full-length cDNA clone encoding a novel isoform of rat inositol 1,4,5-trisphosphate 3-kinase (IP3 3-kinase). Sequence comparison shows it (i) to be 93% identical to human hippocampus IP3 3-kinase B over 468 residues at the protein level, and (ii) to encode a protein 204 amino acids larger than the published sequence of its human homologue.
对大鼠肝脏cDNA表达文库进行免疫筛选，已分离出一个全长cDNA克隆，该克隆编码大鼠肌醇1,4,5 -三磷酸3 -激酶（IP3 3 -激酶）的一种新型同工型。序列比较表明，它（i）在蛋白质水平上与人类海马体IP3 3 -激酶B在468个残基上有93%的同一性，并且（ii）编码的蛋白质比其人类同源物的已发表序列长204个氨基酸。
