Santiago M, Cano J, Westerink B H
Departemento de Bioquimica, Facultad de Farmacia, Sevilla, Spain.
Brain Res. 1993 Nov 19;628(1-2):187-92. doi: 10.1016/0006-8993(93)90954-l.
In the present study, we have infused 6 prototypical drugs known to affect nigrostriatal dopaminergic neurons (kainate, baclofen, muscimol (10 mumol/l), picrotoxin (50 mumol/l), tetrodotoxin (TTX) (5 mumol/l) and 1-methyl-4-phenylpyridinium ion (MPP+) (10 mmol/l) via a microdialysis probe unilaterally into the left substantia nigra. During the infusion of these compounds, the extracellular content of dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) was recorded simultaneously via microdialysis cannulas in both left and right striatum. Intranigral infusion of TTX, MPP+ and baclofen decreased dopamine release in the ipsilateral striatum, whereas muscimol, picrotoxin and kainate increased dopamine release. No changes were seen in the extracellular content of dopamine in the contralateral striatum. During all experiments, extracellular DOPAC increased in the ipsilateral striatum. No changes were seen in the extracellular content of DOPAC in the contralateral striatum. The present data provide no evidence that the bilateral nigrostriatal dopaminergic pathways are functionally linked in the rat brain.
在本研究中，我们通过微透析探针将6种已知可影响黑质纹状体多巴胺能神经元的典型药物（红藻氨酸、巴氯芬、蝇蕈醇（10 μmol/L）、印防己毒素（50 μmol/L）、河豚毒素（TTX）（5 μmol/L）和1-甲基-4-苯基吡啶离子（MPP⁺）（10 mmol/L））单侧注入左侧黑质。在输注这些化合物期间，通过微透析插管同时记录左、右纹状体中多巴胺和3,4-二羟基苯乙酸（DOPAC）的细胞外含量。向黑质内输注TTX、MPP⁺和巴氯芬可降低同侧纹状体中的多巴胺释放，而蝇蕈醇、印防己毒素和红藻氨酸则增加多巴胺释放。对侧纹状体中多巴胺的细胞外含量未见变化。在所有实验中，同侧纹状体中细胞外DOPAC增加。对侧纹状体中DOPAC的细胞外含量未见变化。目前的数据没有提供证据表明双侧黑质纹状体多巴胺能通路在大鼠脑中存在功能联系。
