Yasuhara O, Hanai K, Ohkubo I, Sasaki M, McGeer P L, Kimura H
Institute of Molecular Neurobiology, Shiga University of Medical Science, Otsu, Japan.
Brain Res. 1993 Nov 19;628(1-2):85-92. doi: 10.1016/0006-8993(93)90941-f.
Expression of cystatin C and its mRNA in brain were investigated by use of immunohistochemical and polymerase chain-reaction techniques. High levels of cystatin C mRNA were detected in every region of rat brain examined, including the cerebral cortex, hippocampus, hypothalamus and cerebellum. Cystatin C-positive astrocytes were found by immunohistochemistry to be distributed throughout the brains of rat, monkey and human. Some neurons were also positive, but the staining was weak and variable. Intensely immunoreactive neurons were abundantly found in the cerebral cortex of some aged human cases and of all Alzheimer's disease patients. It is concluded that cystatin C is synthesized and expressed in the central nervous system, especially by astrocytes. Cystatin C might also be involved in the aging process of cortical neurons.
运用免疫组织化学和聚合酶链反应技术，研究了胱抑素C及其mRNA在脑内的表达情况。在检测的大鼠脑的各个区域，包括大脑皮质、海马体、下丘脑和小脑，均检测到高水平的胱抑素C mRNA。通过免疫组织化学发现，胱抑素C阳性星形胶质细胞分布于大鼠、猴和人的全脑。一些神经元也呈阳性，但染色较弱且存在差异。在一些老年人类病例和所有阿尔茨海默病患者的大脑皮质中，大量发现了免疫反应强烈的神经元。得出的结论是，胱抑素C在中枢神经系统中合成并表达，尤其是由星形胶质细胞表达。胱抑素C可能也参与了皮质神经元的衰老过程。
