Tiainen P, Karhi K K
Department of Anesthesiology, Helsinki University Central Hospital, Finland.
Clin Chem. 1994 Feb;40(2):184-9.
Glutathione transferase Alpha (GSTA) is a very sensitive marker of acute centrilobular liver damage. We purified glutathione transferases from human liver and separated the isoenzymes. Polyclonal rabbit antibodies specific for Alpha-class isoenzymes were produced and labeled with Eu(3+)-chelate. We set up a sandwich-type time-resolved immunofluorometric assay (TR-IFMA) to measure GSTA in serum. The detection limit was 0.03 microgram/L, and measuring range was from 0.03 to 100 micrograms/L. The reference range for serum GSTA in this assay was 0.7-6.0 micrograms/L for women and 0.7-14 micrograms/L for men. This TR-IFMA is practical, and the modified rapid assay can be completed in 3 h. Therefore it is applicable for diagnostic purposes in acute liver damage, e.g., associated with drug toxicity or hypoperfusion of the liver.
谷胱甘肽转移酶α（GSTA）是急性小叶中心性肝损伤的一个非常敏感的标志物。我们从人肝脏中纯化谷胱甘肽转移酶并分离同工酶。制备了对α类同工酶特异的兔多克隆抗体并用铕（3+）螯合物进行标记。我们建立了一种夹心型时间分辨免疫荧光测定法（TR-IFMA）来检测血清中的GSTA。检测限为0.03微克/升，测量范围为0.03至100微克/升。该测定法中血清GSTA的参考范围女性为0.7 - 6.0微克/升，男性为0.7 - 14微克/升。这种TR-IFMA实用性强，改进后的快速测定法可在3小时内完成。因此它适用于急性肝损伤的诊断，例如与药物毒性或肝脏低灌注相关的急性肝损伤。
