Cavaliere F, Guadagni F, D'Agnano I, Casaldi V, Sciarretta F, Spila A, Cosimelli M
Department of Surgery, Regina Elena Cancer Institute, Rome, Italy.
Dis Colon Rectum. 1994 Feb;37(2 Suppl):S24-9. doi: 10.1007/BF02048427.
The aim of this study was to evaluate the possibility of identifying biologically aggressive subgroups of patients, combining several biologic parameters such as the tumor and normal mucosa values from the ploidy, the S-phase cell percentage, and tumor-associated glycoprotein-72 (TAG-72) expression.
One hundred five colorectal cancer patients were studied to analyze the possible prognostic role of ploidy and cell kinetics in multiple fresh specimens from the tumor and normal mucosa. Since the presence of TAG-72 in the colonic mucosa has been correlated to neoplastic transformation, the correlations between these parameters and the quantitative tissue expression of the TAG-72 were analyzed in a subgroup of 53 cases.
A significantly lower five-year disease-free survival rate (21.4 percent) was observed in patients with multiploid tumors, when compared with that observed in patients with diploid or single aneuploid tumors (67.5 percent) (P = 0.03). The quantitative tissue TAG-72 expression contributed in identifying a particular patient subgroup (20 percent), characterized by S-phase percentage and TAG-72 values from the normal mucosa that were unexpectedly higher than 12.1 percent and 7.5 U/mg of proteins, respectively. In particular, when the 25 Dukes B patients were analyzed, similar results were observed. In fact, 14 (56 percent) cases showed high tumor cell proliferation and, surprisingly, a high tissue TAG-72 content in the normal mucosa was found in 4 (28.6 percent) of these patients.
Other than multiploidy, the biologic aggressiveness of colorectal cancer might be successfully assessed introducing the evaluation of new biologic parameters, such as the TAG-72 content and S-phase percentage values of the normal mucosa, suggesting the possibility of further stratifying this patient population.
本研究旨在评估结合多种生物学参数（如来自倍体、S期细胞百分比以及肿瘤相关糖蛋白72（TAG - 72）表达的肿瘤和正常黏膜值）来识别具有生物学侵袭性的患者亚组的可能性。
对105例结直肠癌患者进行研究，分析倍体和细胞动力学在来自肿瘤和正常黏膜的多个新鲜标本中的可能预后作用。由于结肠黏膜中TAG - 72的存在与肿瘤转化相关，在53例患者的亚组中分析了这些参数与TAG - 72定量组织表达之间的相关性。
与二倍体或单非整倍体肿瘤患者（67.5%）相比，多倍体肿瘤患者的五年无病生存率显著更低（21.4%）（P = 0.03）。定量组织TAG - 72表达有助于识别一个特定的患者亚组（20%），其特征为S期百分比以及正常黏膜的TAG - 72值分别意外高于12.1%和7.5 U/mg蛋白质。特别是，当分析25例Dukes B期患者时，观察到了类似结果。实际上，14例（56%）病例显示肿瘤细胞增殖高，并且令人惊讶的是，其中4例（28.6%）患者的正常黏膜中发现了高组织TAG - 72含量。
除了多倍体之外，通过引入新的生物学参数（如正常黏膜的TAG - 72含量和S期百分比值）的评估，可能成功评估结直肠癌的生物学侵袭性，这表明进一步对该患者群体进行分层的可能性。
