谷氨酰胺对肝癌组织培养细胞中谷氨酰胺合成酶降解的影响。
Effect of glutamine on the degradation of glutamine synthetase in hepatoma tissue-culture cells.
作者信息
Crook R B, Tomkins G M
出版信息
Biochem J. 1978 Oct 15;176(1):47-52. doi: 10.1042/bj1760047.
Abstract
In certain lines of hepatoma tissue-culture cells, the extracellular glutamine concentration regulates the specific activity of glutamine synthetase. By quantifying the radioactivity in immunoprecipitated glutamine synthetase on polyacrylamide gels, we found that the rate of degradation, but not of synthesis, of glutamine synthetase is a sensitive function of extracellular glutamine. The activiy that degrades this enzyme appears to be labile.
摘要
在某些肝癌组织培养细胞系中，细胞外谷氨酰胺浓度调节谷氨酰胺合成酶的比活性。通过对聚丙烯酰胺凝胶上免疫沉淀的谷氨酰胺合成酶中的放射性进行定量，我们发现谷氨酰胺合成酶的降解速率而非合成速率是细胞外谷氨酰胺的敏感函数。降解该酶的活性似乎不稳定。
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