Rose B R, Thompson C H, Jiang X M, Tattersall M H, Elliott P M, Dalrymple C, Cossart Y E
Department of Infectious Diseases, University of Sydney, New South Wales, Australia.
Gynecol Oncol. 1994 Feb;52(2):212-7. doi: 10.1006/gyno.1994.1033.
Human papillomavirus (HPV) 16 E6/E7 mRNAs have been detected in paraffin-embedded sections of histologically cancer-free pelvic lymph nodes from four of six patients with HPV 16-associated cervical cancer. The cDNA obtained from the viral mRNA by reverse transcription was amplified by the polymerase chain reaction (PCR). Transcripts were present in a small but significant proportion (6/42, 14%) of the histologically negative/HPV 16 DNA-positive lymph node blocks; but neither HPV 16 DNA nor transcripts were found in 12 lymph node blocks from two patients whose cervical cancers were not HPV-related. Both of the HPV 16 mRNAs detectable by the PCR primers used in the assay (the E6*I and the full-length E6 transcript) were found in the primary tumors, but the transcription patterns in the lymph nodes were variable. The presence of HPV E6/E7 mRNAs in lymph nodes of patients with HPV-related cancer may be a more sensitive indicator of metastasis than conventional histology. In addition, their detections is likely to be more significant than that of HPV DNA sequences alone. The practical significance of the findings, however, awaits correlation with the ultimate clinical outcome.
在6例人乳头瘤病毒16型（HPV - 16）相关宫颈癌患者中，有4例患者组织学检查无癌的盆腔淋巴结石蜡包埋切片中检测到了HPV - 16 E6/E7信使核糖核酸（mRNA）。通过逆转录从病毒mRNA获得的互补DNA（cDNA），用聚合酶链反应（PCR）进行扩增。在组织学检查为阴性/HPV - 16 DNA阳性的淋巴结组织块中，转录本存在于一小部分但比例显著的样本中（6/42，14%）；而在两名宫颈癌与HPV无关患者的12个淋巴结组织块中，未发现HPV - 16 DNA和转录本。检测中使用的PCR引物可检测到的两种HPV - 16 mRNA（E6*I和全长E6转录本）在原发肿瘤中均有发现，但淋巴结中的转录模式各不相同。HPV相关癌症患者淋巴结中HPV E6/E7 mRNA的存在可能是比传统组织学更敏感的转移指标。此外，它们的检测可能比单独检测HPV DNA序列更有意义。然而，这些发现的实际意义还有待与最终临床结果相关联。
