Wagner T M, Adler A, Sevelda P, Assmann I, Knepflé C F, Czerwenka K, Heinzl H, Ploem-Zaaijer J J
Department of Cytochemistry and Cytometry, Sylvius Laboratory, University of Leiden, The Netherlands.
Int J Cancer. 1994 Jan 15;56(2):167-72. doi: 10.1002/ijc.2910560203.
Paraffin-embedded material from 69 patients with epithelial ovarian cancer FIGO stages I and II/A (including 21 patients with borderline carcinoma) was studied with automatic DNA image cytometry. Univariate analysis indicated a significant difference in survival based on the presence of nuclei with high DNA content (higher than 5 C). A group of patients with less than 0.2% cells with high DNA content had a 6-year survival of 87%, whereas in a group of patients with more than 0.2% of such cells, 6-year survival was 49%. This parameter remained significant when used in a group of stage I/a and I/b patients. Statistical analysis of diploid vs. non-diploid tumors also showed significant difference in survival. Separate analysis of 48 invasive ovarian cancers indicated that ploidy, the percentage of cells with high DNA content and tumor stage (stage I/a + b vs. stages I/c + II/a) reached significance for survival, whereas grading did not. In addition, comparison of clinical stage, grading, ploidy and the percentage of cells exceeding 5 C with a threshold at 0.2% by means of a multivariate analysis (Cox regression model) showed that only the percentage of cells exceeding 5 C remained statistically significant.
对69例国际妇产科联盟（FIGO）I期和II/A期上皮性卵巢癌患者（包括21例交界性癌患者）的石蜡包埋材料进行了自动DNA图像细胞术研究。单因素分析表明，基于高DNA含量（高于5C）细胞核的存在，生存率存在显著差异。一组DNA含量高的细胞少于0.2%的患者6年生存率为87%，而在一组此类细胞超过0.2%的患者中，6年生存率为49%。当用于I/a期和I/b期患者组时，该参数仍然具有显著性。二倍体肿瘤与非二倍体肿瘤的生存统计分析也显示出生存率的显著差异。对48例浸润性卵巢癌的单独分析表明，倍体、DNA含量高的细胞百分比和肿瘤分期（I/a + b期与I/c + II/a期）对生存率有显著意义，而分级则无显著意义。此外，通过多因素分析（Cox回归模型）比较临床分期、分级、倍体和超过5C的细胞百分比（阈值为0.2%），结果显示只有超过5C的细胞百分比仍具有统计学显著性。
