Roberts R G, Coffey A J, Bobrow M, Bentley D R
Paediatric Research Unit, Division of Medical & Molecular Genetics, London, United Kingdom.
Genomics. 1993 May;16(2):536-8. doi: 10.1006/geno.1993.1225.
Application of a novel vectorette PCR approach to defining intron-exon boundaries has permitted completion of analysis of the exon structure of the largest and most complex known human gene. We present here a summary of the exon structure of the entire human dystrophin gene, together with the sizes of genomic HindIII fragments recognized by each exon, and (where available) GenBank accession numbers for adjacent intron sequences.
一种新型的载体PCR方法在确定内含子-外显子边界方面的应用,使得对已知最大且最复杂的人类基因的外显子结构分析得以完成。我们在此呈现整个人类肌营养不良蛋白基因外显子结构的总结,以及每个外显子所识别的基因组HindIII片段的大小,还有(如有可用)相邻内含子序列的GenBank登录号。