Vollmers H P, von Landenberg P, Dämmrich J, Stulle K, Wozniak E, Ringdörfer C, Müller-Hermelink H K, Herrmann B, Zimmermann U
Institut für Pathologie, Universität Würzburg, Germany.
Hybridoma. 1993 Apr;12(2):221-5. doi: 10.1089/hyb.1993.12.221.
The advantages of electrofusion were used to immortalize the small number of B-cells from fresh biopsy material taken from a gastric carcinoma of a patient. Two stable human antibody secreting clones could be produced which exhibited functional activity against the autologous tumour cells (inhibition of cell adhesion and immunofluorescence staining of the membranes). This shows that a variety of hitherto inaccessible B lymphocyte populations from other human organ biopsies can be immortalised by the improved electrofusion technique.
利用电融合的优势，将取自一名胃癌患者新鲜活检材料中的少量B细胞永生化。可产生两个稳定的分泌人抗体的克隆，它们对自体肿瘤细胞表现出功能活性（抑制细胞粘附和细胞膜的免疫荧光染色）。这表明，通过改进的电融合技术，可以使来自其他人体器官活检的各种迄今难以获得的B淋巴细胞群体永生化。
