Klebanoff S J, Schlechte K G, Waltersdorph A M
Department of Medicine, University of Washington, Seattle 98195.
J Leukoc Biol. 1993 Jun;53(6):666-72. doi: 10.1002/jlb.53.6.666.
An antioxidant effect of manganese (Mn) complexes due to the scavenging of the superoxide anion (O2-.) or to the decomposition of hydrogen peroxide (H2O2) has been described. We report here that Mn also can exert a prooxidant effect under certain experimental conditions. Thus Mn2+ in phosphate buffer increased the bactericidal effect of phorbol myristate acetate-stimulated polymorphonuclear leukocytes (PMNs) on extracellular Escherichia coli. This effect was inhibited by azide, catalase, and a decrease in chloride concentration and was not observed when normal PMNs were replaced by those of patients with chronic granulomatous disease or myeloperoxidase (MPO) deficiency. Mn2+ could be replaced by Mn3+ or by superoxide dismutase (SOD). These findings suggest that Mn (or SOD), by increasing the conversion of O2-. to H2O2, can increase the activity of the MPO-H2O2-chloride antimicrobial system released by stimulated PMNs.
锰（Mn）配合物通过清除超氧阴离子（O2-.）或分解过氧化氢（H2O2）而具有抗氧化作用，这一点已有报道。我们在此报告，在某些实验条件下，Mn也可发挥促氧化作用。因此，磷酸盐缓冲液中的Mn2+增强了佛波酯肉豆蔻酸酯刺激的多形核白细胞（PMN）对细胞外大肠杆菌的杀菌作用。叠氮化物、过氧化氢酶以及氯化物浓度降低可抑制此作用，而当正常PMN被慢性肉芽肿病患者或髓过氧化物酶（MPO）缺乏患者的PMN替代时则未观察到此作用。Mn2+可被Mn3+或超氧化物歧化酶（SOD）替代。这些发现表明，Mn（或SOD）通过增加O2-.向H2O2的转化，可增强受刺激PMN释放的MPO-H2O2-氯化物抗菌系统的活性。
