Lu X, Richardson P M
Division of Neurosurgery, McGill University, Montreal, Canada.
J Neurocytol. 1993 May;22(5):334-41. doi: 10.1007/BF01195557.
Immunohistochemical studies with monoclonal antibodies to macrophage antigens were performed on sections of rat lumbar dorsal root ganglia. In confirmation of previous observations, cells with macrophage antigenicity were detected in normal ganglia. Many of these presumptive macrophages were perineuronal in contact with the neuron/satellite cell complex, a few were perivascular, and others were in interstitial position not in apparent contact with either blood vessels or neurons. The number of macrophages in lumbar dorsal root ganglia started to increase 2-4 days after sciatic nerve transection and remained elevated for four weeks. Perineuronal macrophages resembled satellite glial cells in light microscope appearance but were distinguished from glial cells by their lack of S-100 immunoreactivity. Following this sciatic nerve injury, macrophage counts were modestly increased in contralateral lumbar dorsal root ganglia but not in cervical dorsal root ganglia. Thus peripheral nerve injury induces a recruitment and/or proliferation of macrophages in the corresponding dorsal root ganglion. Although the functions of these macrophages are unclear, those in perineuronal position could contribute to the survival or regeneration of axotomized neurons.
利用抗巨噬细胞抗原的单克隆抗体对大鼠腰段背根神经节切片进行了免疫组织化学研究。证实了先前的观察结果，即在正常神经节中检测到具有巨噬细胞抗原性的细胞。这些推测的巨噬细胞许多位于神经元周围，与神经元/卫星细胞复合体接触，少数位于血管周围，其他的则处于间质位置，与血管或神经元均无明显接触。坐骨神经横断后2 - 4天，腰段背根神经节中的巨噬细胞数量开始增加，并持续升高四周。神经元周围的巨噬细胞在光学显微镜下的外观与卫星胶质细胞相似，但通过缺乏S - 100免疫反应性与胶质细胞区分开来。坐骨神经损伤后，对侧腰段背根神经节中的巨噬细胞计数略有增加，但颈段背根神经节中则没有。因此，外周神经损伤可诱导相应背根神经节中巨噬细胞的募集和/或增殖。虽然这些巨噬细胞的功能尚不清楚，但位于神经元周围的巨噬细胞可能有助于轴突切断的神经元的存活或再生。
