Britton C, Canto G J, Urquhart G M, Kennedy M W
Wellcome Laboratories for Experimental Parasitology, University of Glasgow, UK.
Parasite Immunol. 1993 Mar;15(3):163-74. doi: 10.1111/j.1365-3024.1993.tb00596.x.
In vitro released products of the adult stage of the bovine lungworm. Dictyocaulus viviparus, were characterized according to their SDS-PAGE profile, glycosylation pattern, in vitro synthesis and antigenicity in the context of infection and vaccination with irradiated larvae. Biosynthetic labelling experiments with 35S-methionine indicated active synthesis of ES throughout this time. There was, however, little incorporation of 3H-glucosamine into ES products, and lectin affinity chromatography and glycopeptidase F digestion identified only one glycosylated component. Immunoprecipitation of 125I-labelled ES products with sera from calves patently infected with D. viviparus demonstrated that all of these, with the exception of two components, are antigenic to the bovine host. One of those not immunoprecipitated was shown to be host serum albumin carried over into culture. A limited degree of cross-reactivity between nematode species was observed, with a D. viviparus female-specific antigen of 290 kDa being recognized by serum antibody from calves infected with the gastrointestinal nematodes Cooperia oncophora and Ostertagia ostertagi. Calves vaccinated with irradiated larvae of D. viviparus, despite not being exposed to the adult stage of the parasite, also showed some recognition of adult ES products. This might suggest that vaccination with irradiated larvae operates against both pre-pulmonary and pulmonary stages of the infection.
对牛肺线虫成虫阶段（胎生网尾线虫）的体外释放产物，根据其十二烷基硫酸钠-聚丙烯酰胺凝胶电泳图谱、糖基化模式、体外合成情况以及在受辐照幼虫感染和疫苗接种背景下的抗原性进行了表征。用³⁵S-甲硫氨酸进行的生物合成标记实验表明，在此期间整个过程中排泄分泌产物（ES）都有活跃合成。然而，³H-葡糖胺很少掺入到ES产物中，凝集素亲和层析和糖肽酶F消化仅鉴定出一种糖基化成分。用明显感染胎生网尾线虫的小牛血清对¹²⁵I标记的ES产物进行免疫沉淀，结果表明，除了两个成分外，所有这些产物对牛宿主都具有抗原性。其中一个未被免疫沉淀的成分被证明是带入培养物中的宿主血清白蛋白。观察到线虫物种之间存在有限程度的交叉反应，感染胃肠道线虫柯柏线虫和奥斯特他线虫的小牛血清抗体可识别胎生网尾线虫290 kDa的雌性特异性抗原。用胎生网尾线虫辐照幼虫接种的小牛，尽管未接触到该寄生虫的成虫阶段，但也对成虫ES产物有一定的识别。这可能表明用辐照幼虫进行疫苗接种对感染的肺前期和肺期均有作用。
