Bradley J E, Gillespie A J, Trenholme K R, Karam M
Department of Biology, Imperial College of Science, Technology and Medicine, London.
Parasitology. 1993 May;106 ( Pt 4):363-70. doi: 10.1017/s003118200006710x.
The effects of exposure to infective larvae on the antibody response to a cocktail of specific recombinant antigens of Onchocerca volvulus and to a worm extract were evaluated by comparing the responses of individuals from a vector controlled area with those from an area of continuing transmission by ELISA. Individuals from the vector controlled areas were found to have reduced responses to both antigen preparations. A microfilerdermic (mf-) individuals from the area of vector control exhibited significantly lower total and subclass IgG responses to the worm extract. In contrast, the responses to the cocktail of specific recombinants were significantly reduced in individuals from the area of vector control who were still microfilerdermia positive (mf+). The distribution of IgG subclass specific responses was similar to both antigen preparations, both dominated by the IgG4 and IgG1 subclasses. IgG1 responses to the worm extract remained elevated in the vector controlled individuals but IgG4 was significantly reduced in the mf- individuals. Both subclasses reflected the total IgG response to the cocktail of recombinants and were significantly reduced in individuals from the vector controlled area, when compared to individuals from the hyperendemic area. IgG1 responses to the cocktail of recombinants are significantly lower than IgG4 in all individuals and virtually absent in individuals from the vector-controlled area. Measuring total IgG and IgG4 is more sensitive than IgG1 in detecting infection, 100 or 97% respectively, but they remain elevated in the individuals from the vector controlled areas even after 8-10 years interruption of transmission. These results have important implications for the serological monitoring of control programmes in individuals who have previously been infected.
通过酶联免疫吸附测定法（ELISA）比较来自病媒控制地区的个体与持续传播地区个体的反应，评估暴露于感染性幼虫对盘尾丝虫特定重组抗原混合物及虫体提取物抗体反应的影响。发现来自病媒控制地区的个体对两种抗原制剂的反应均降低。来自病媒控制地区的微丝蚴血症（mf-）个体对虫体提取物的总IgG及亚类IgG反应显著降低。相比之下，来自病媒控制地区仍为微丝蚴血症阳性（mf+）的个体对特定重组抗原混合物的反应显著降低。对两种抗原制剂的IgG亚类特异性反应分布相似，均以IgG4和IgG1亚类为主。病媒控制个体中对虫体提取物的IgG1反应仍较高，但mf-个体中的IgG4显著降低。与高度流行地区的个体相比，两个亚类均反映了对重组抗原混合物的总IgG反应，且来自病媒控制地区个体的该反应显著降低。在所有个体中，对重组抗原混合物的IgG1反应均显著低于IgG4，在病媒控制地区个体中几乎检测不到。检测感染时，测量总IgG和IgG4分别比IgG1更敏感，分别为100%或97%，但即使在传播中断8至10年后，病媒控制地区个体中的这些指标仍保持较高水平。这些结果对先前感染个体的控制项目血清学监测具有重要意义。
