Rueff A, Dray A
Sandoz Institute for Medical Research, London.
Agents Actions. 1993;38 Spec No:C13-5. doi: 10.1007/BF01991122.
The excitatory and sensitizing properties of 5-HT and different prostaglandins (PGD2, PGE1, PGE2, PGF2 alpha, PGI2 and PGI2-analogue, cicaprost) were characterized on an in vitro preparation of the neonatal rat spinal cord with functionally attached tail. Prolonged (10 min) perfusion of the tail with 5-hydroxy-tryptamine (5-HT, 0.5-10 microM) or any of the tested prostaglandins (0.1-5 microM) did not evoke an excitatory response recorded from a lumbar ventral root, but significantly enhanced responses of peripheral nociceptors to thermal and chemical (bradykinin, capsaicin) stimuli. PGD2 did not induce such an enhancement. Following sensitization of peripheral nociceptors with low concentrations of bradykinin or capsaicin, 5-HT (1-10 microM) evoked a ventral root response. Using specific 5-HT-receptor agonists and antagonists, 5-HT-evoked excitation was determined to be mediated via a 5-HT1-like receptor while 5-HT-induced sensitization involved 5-HT2 receptors.
利用连接有功能性尾巴的新生大鼠脊髓体外制备物，对5-羟色胺（5-HT）和不同前列腺素（PGD2、PGE1、PGE2、PGF2α、PGI2及PGI2类似物西卡前列素）的兴奋性和致敏特性进行了表征。用5-羟色胺（5-HT，0.5 - 10微摩尔）或任何一种受试前列腺素（0.1 - 5微摩尔）对尾巴进行长时间（10分钟）灌注，并未引发从腰段腹根记录到的兴奋性反应，但显著增强了外周伤害感受器对热刺激和化学刺激（缓激肽、辣椒素）的反应。PGD2并未诱导这种增强作用。在用低浓度缓激肽或辣椒素使外周伤害感受器致敏后，5-HT（1 - 10微摩尔）引发了腹根反应。使用特异性5-HT受体激动剂和拮抗剂，确定5-HT引发的兴奋是通过5-HT1样受体介导的，而5-HT诱导的致敏涉及5-HT2受体。
