Morris S J, Schober R, Schultens H A
Biochim Biophys Acta. 1977 Jan 4;464(1):65-81. doi: 10.1016/0005-2736(77)90371-6.
Exchanging 0.3 M sucrose for 0.3 M glycerol as the bathing medium for isolated bovine adrenal chromaffin granules at 0 degrees C or raising the temperature of granules suspended in 0.3 M sucrose to 37 degrees C causes the release of catecholamines, ATP and soluble protein stored in the granules. These treatments also reduce the turbidity (absorbance at a non-absorbing wavelength) of the same suspensions. The glycerol-induced release is shown by morphology and morphometry to be due to lysis of the granules. Advantage is taken of the difference in density of the intact granules and the granule ghosts to confirm this result by centrifugation of glycerol-incubated samples on continuous gradients of sucrose and metrizamide. Similar centrifugation experiments performed on the granules incubated in sucrose at 37 degrees C show that lysis also accounts for the fast phase of release of granule contents, and not graded selective release of material from otherwise intact granules.
在0℃下，将分离的牛肾上腺嗜铬颗粒的浴液介质由0.3M蔗糖换成0.3M甘油，或将悬浮于0.3M蔗糖中的颗粒温度升至37℃，会导致颗粒中储存的儿茶酚胺、ATP和可溶性蛋白质释放出来。这些处理还会降低相同悬浮液的浊度（在非吸收波长处的吸光度）。通过形态学和形态计量学表明，甘油诱导的释放是由于颗粒的裂解。利用完整颗粒和颗粒“幽灵”（ghost，指裂解后的颗粒残余部分）密度的差异，通过在蔗糖和甲泛影酰胺连续梯度上对甘油孵育样品进行离心来证实这一结果。对在37℃蔗糖中孵育的颗粒进行的类似离心实验表明，裂解也解释了颗粒内容物快速释放的阶段，而不是从其他方面完整的颗粒中分级选择性释放物质。
