用地高辛标记探针原位杂交法对马骨骼肌中黄嘌呤脱氢酶mRNA进行定位。
Localization of xanthine dehydrogenase mRNA in horse skeletal muscle by in situ hybridization with digoxigenin-labelled probe.
作者信息
Räsänen L A, Karvonen U, Pösö A R
机构信息
Department of Biochemistry, College of Veterinary Medicine, Helsinki, Finland.
出版信息
Biochem J. 1993 Jun 15;292 ( Pt 3)(Pt 3):639-41. doi: 10.1042/bj2920639.
Abstract
In situ hybridization was used to localize xanthine dehydrogenase (XDH) mRNA in horse skeletal muscle. Capillary endothelial cells were found to express XDH, but muscle cells did not give any signal. The digoxigenin-labelled probe was produced by PCR with primers based on the cDNA sequence of mouse XDH and horse lung cDNAs. A 4.3 kb mRNA was detected in a Northern blot.
摘要
采用原位杂交技术在马骨骼肌中定位黄嘌呤脱氢酶（XDH）mRNA。发现毛细血管内皮细胞表达XDH，但肌细胞未给出任何信号。地高辛标记的探针通过基于小鼠XDH cDNA序列和马肺cDNA的引物进行PCR制备。在Northern印迹中检测到一条4.3 kb的mRNA。
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