Kuyatt B L, Reidy C A, Hui K Y, Jordan W H
Eli Lilly & Co., Toxicology Research Center, Greenfield, Indiana 46140.
Anal Quant Cytol Histol. 1993 Apr;15(2):83-7.
Color image analysis was used to assess proliferative changes in smooth muscle cells from cultured segments of rabbit aortas. Proliferating cells were labeled with bromodeoxyuridine (BrdU) and visualized by immunohistochemical staining of histologic sections. A Macintosh IIfx computer with a Data Translation digitizer board, Javelin color camera and a color-enhanced version of National Institutes of Health Image 1.31 image analysis software (ColorImage 1.31) was used to acquire red, green and blue (RGB)-filtered grayscale images from microscopic slides of control and treated aortas. The BrdU-labeled (brown) and nonlabeled, hematoxylin (blue)-stained nuclei were identified on the RGB gray-scale images using a thresholding technique and sampled for nuclear number and area. An increase in the number of BrdU-labeled nuclei in the region of experimental perturbation was demonstrated by this semiautomated method. Thus, this Macintosh-based color image analysis method proved to be effective in rapidly quantitating immunohistochemically defined smooth muscle proliferation in microscopic tissues.
彩色图像分析用于评估兔主动脉培养节段中平滑肌细胞的增殖变化。增殖细胞用溴脱氧尿苷（BrdU）标记，并通过组织学切片的免疫组织化学染色进行可视化。使用配备Data Translation数字化仪板、标枪彩色相机和美国国立卫生研究院图像1.31图像分析软件（ColorImage 1.31）彩色增强版的Macintosh IIfx计算机，从对照和处理过的主动脉显微镜载玻片上获取红色、绿色和蓝色（RGB）滤过的灰度图像。使用阈值技术在RGB灰度图像上识别BrdU标记（棕色）和未标记的苏木精（蓝色）染色细胞核，并对细胞核数量和面积进行采样。这种半自动化方法证明了实验扰动区域中BrdU标记细胞核数量的增加。因此，这种基于Macintosh的彩色图像分析方法被证明可有效地快速定量微观组织中免疫组织化学定义的平滑肌增殖。
