Ao A, Erickson R P, Stalvey J R
Department of Pediatrics, Steele Memorial Children's Research Center, University of Arizona Health Science Center, University of Arizona, Tucson 85724.
Mol Reprod Dev. 1993 Jun;35(2):159-64. doi: 10.1002/mrd.1080350209.
We have examined the transcription of Müllerian inhibiting substance (MIS) in testis by the sensitive technique of reverse transcription polymerase chain reaction (RTPCR). A developmental study of testis by this nonquantitative technique showed expression at all postnatal stages, including adults while liver and kidney provided negative controls. Cell separation studies indicated that highly purified interstitial cells, as well as less homogeneous Sertoli cell-enriched and germ cell-enriched fractions, contained RNA for MIS. The transcription of MIS in an interstitial cell type was confirmed by finding MIS mRNA in purified Leydig cells. Inasmuch as the germ cell-enriched fraction contains some Sertoli cells, and XX,Sxra and XX,Sxrb which have germ cell-depleted testes, contain MIS mRNA, a Sertoli cell source remains likely for the seminiferous tubule compartment.
我们采用逆转录聚合酶链反应（RTPCR）这一灵敏技术检测了睾丸中苗勒管抑制物质（MIS）的转录情况。通过这种非定量技术对睾丸进行的发育研究表明，在包括成年期在内的所有出生后阶段均有表达，而肝脏和肾脏作为阴性对照。细胞分离研究表明，高度纯化的间质细胞，以及不太均一的富含支持细胞和富含生殖细胞的组分，都含有MIS的RNA。在纯化的睾丸间质细胞中发现MIS mRNA，证实了间质细胞类型中MIS的转录。由于富含生殖细胞的组分含有一些支持细胞，并且XX、Sxra和XX、Sxrb这些睾丸生殖细胞缺失的个体也含有MIS mRNA，所以支持细胞仍可能是生精小管部分的来源。
