Logothetis D E, Kammen B F, Lindpaintner K, Bisbas D, Nadal-Ginard B
Howard Hughes Medical Institute, Department of Cardiology, Children's Hospital Medical Center, Boston, Massachusetts.
Neuron. 1993 Jun;10(6):1121-9. doi: 10.1016/0896-6273(93)90060-5.
Voltage-gated ion channels that differ in their primary amino acid sequence in the putative voltage sensor, the S4 region, show distinct voltage-sensing characteristics. In this study, we directly compared two voltage-gated K+ channels, the mammalian RCK1 with the Drosophila Shab11, and correlated the specific amino acid content of their respective S4 regions with the distinct voltage-sensing properties they exhibit. We find that specific differences in the charge content of the S4 region are sufficient to account for the distinct gating valence of each channel. However, differences in residues inside the S4 region are not sufficient to account for each channel's characteristic voltage range of activation.
在假定的电压感受器S4区域中，其一级氨基酸序列不同的电压门控离子通道表现出不同的电压传感特性。在本研究中，我们直接比较了两种电压门控钾通道，即哺乳动物的RCK1和果蝇的Shab11，并将它们各自S4区域的特定氨基酸含量与其所表现出的不同电压传感特性相关联。我们发现，S4区域电荷含量的特定差异足以解释每个通道不同的门控价。然而，S4区域内部残基的差异不足以解释每个通道的特征性激活电压范围。
