肝素在聚丙烯酸酯基微珠上的非特异性吸附和共价偶联。
Nonspecific adsorption and covalent coupling of heparin on polyacrylate based microbeads.
作者信息
Denizli A, Kiremitçi M, Pişkin E
机构信息
Chemical Engineering Department, Hacettepe University, Beytepe, Ankara, Turkey.
出版信息
Biomater Artif Cells Immobilization Biotechnol. 1993;21(2):183-98. doi: 10.3109/10731199309117356.
Polyacrylate based microbeads were prepared by copolymerization of four different acrylate monomers, namely 2-hydroxyethylmethacrylate (HEMA), ethyleneglycoldimethacrylate (EGDMA), methylmethacrylate (MMA) and dimethylaminoethylmethacrylate (DMEAMA). These beads were further activated with CNBr at alkaline pH. The extend of nonspecific adsorption and covalent coupling of heparin on these beads were investigated in a batch reactors at different temperatures. The effects of initial concentrations of activation agent and heparin were also studied. Nonspecific heparin adsorption on the microbeads containing DMAEMA was significantly higher than the others. Nonspecific adsorption decreased with increasing temperature. Heparin was covalently coupled on CNBr activated microbeads. The amount of coupled heparin increased by increasing concentration of CNBr.
基于聚丙烯酸酯的微珠通过四种不同丙烯酸酯单体的共聚制备，这四种单体分别是甲基丙烯酸2-羟乙酯（HEMA）、乙二醇二甲基丙烯酸酯（EGDMA）、甲基丙烯酸甲酯（MMA）和甲基丙烯酸二甲氨基乙酯（DMEAMA）。这些珠子在碱性pH下用溴化氰进一步活化。在间歇式反应器中于不同温度下研究了肝素在这些珠子上的非特异性吸附和共价偶联程度。还研究了活化剂和肝素初始浓度的影响。肝素在含有DMAEMA的微珠上的非特异性吸附显著高于其他微珠。非特异性吸附随温度升高而降低。肝素共价偶联在溴化氰活化的微珠上。通过增加溴化氰浓度，偶联的肝素量增加。
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