Gomaa Z S
Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Biomed Chromatogr. 1993 May-Jun;7(3):134-5. doi: 10.1002/bmc.1130070305.
A high performance liquid chromatographic assay for the quantitation of acetazolamide in both tablet and injection form is described. Acetazolamide is extracted with 0.005 M NaOH solution containing 0.3 mg/mL sulphadiazine (internal standard). A commercially available mu-Bondapak C18 cartridge column was used for the separation together with a mobile phase made of acetonitrile, methanol and sodium acetate buffer mixture (10:2:88) (pH 4) at a flow-rate of 4 mL/min. Retention times of about 2.50 and 3.36 min were obtained for the drug and the internal standard, respectively.
本文描述了一种用于定量片剂和注射剂形式乙酰唑胺的高效液相色谱法。乙酰唑胺用含有0.3mg/mL磺胺嘧啶（内标）的0.005M氢氧化钠溶液萃取。使用市售的μ-Bondapak C18柱进行分离，流动相由乙腈、甲醇和醋酸钠缓冲液混合物（10:2:88）（pH 4）组成，流速为4mL/min。药物和内标的保留时间分别约为2.50分钟和3.36分钟。
