Cloning and expression of chicken skeletal muscle troponin I in Escherichia coli: the role of rare codons on the expression level.
作者信息
Quaggio R B, Ferro J A, Monteiro P B, Reinach F C
机构信息
Departamento de Bioquímica, Universidade de São Paulo, Brazil.
出版信息
Protein Sci. 1993 Jun;2(6):1053-6. doi: 10.1002/pro.5560020618.
Abstract
摘要
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The interaction of the calcium-binding protein (troponin C) with bivalent cations and the inhibitory protein (troponin I).钙结合蛋白（肌钙蛋白C）与二价阳离子及抑制蛋白（肌钙蛋白I）之间的相互作用。
Biochem J. 1974 Feb;137(2):145-54. doi: 10.1042/bj1370145.
2
Reconstitution of troponin activity from three protein components.由三种蛋白质成分重构肌钙蛋白活性。
J Biol Chem. 1971 Jul 10;246(13):4226-33.
3
The relaxing protein system of striated muscle. Resolution of the troponin complex into inhibitory and calcium ion-sensitizing factors and their relationship to tropomyosin.横纹肌的舒张蛋白系统。肌钙蛋白复合体分解为抑制因子和钙离子敏感因子及其与原肌球蛋白的关系。
Biochem J. 1969 Dec;115(5):993-1004. doi: 10.1042/bj1150993.
4
Structure of the calcium regulatory muscle protein troponin-C at 2.8 A resolution.钙调节肌肉蛋白肌钙蛋白-C在2.8埃分辨率下的结构。
Nature. 1985;313(6004):653-9. doi: 10.1038/313653a0.
5
Molecular structure of troponin C from chicken skeletal muscle at 3-angstrom resolution.鸡骨骼肌肌钙蛋白C在3埃分辨率下的分子结构。
Science. 1985 Feb 22;227(4689):945-8. doi: 10.1126/science.3969570.
6
The AGG codon is translated slowly in E. coli even at very low expression levels.即使在极低的表达水平下，AGG密码子在大肠杆菌中也翻译得很慢。
Nucleic Acids Res. 1988 Apr 11;16(7):3013-24. doi: 10.1093/nar/16.7.3013.
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Cloning, sequencing and expression of a full-length rabbit fast skeletal troponin-C cDNA.兔快肌肌钙蛋白-C全长cDNA的克隆、测序及表达
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The chicken fast skeletal troponin I gene: exon organization and sequence.鸡快速骨骼肌肌钙蛋白I基因：外显子结构与序列
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Synthesis of a troponin C cDNA and expression of wild-type and mutant proteins in Escherichia coli.
J Biol Chem. 1988 Sep 25;263(27):13962-9.
10
Cloning, expression, and site-directed mutagenesis of chicken skeletal muscle troponin C.鸡骨骼肌肌钙蛋白C的克隆、表达及定点诱变
J Biol Chem. 1988 Feb 15;263(5):2371-6.
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