Sarmah S, More T
Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar.
Comp Immunol Microbiol Infect Dis. 1993 Apr;16(2):173-8. doi: 10.1016/0147-9571(93)90010-3.
Due to lack of studies on PMN granular cationic proteins to assess immunostatus of buffaloes, the peripheral PMN leukocytes were subjected to homogenization by sonication and centrifugation cycles in order to obtain pure pellet of granules. Acid extracted granules contained cationic proteins and peptides, which were further separated by exclusion Sephadex G-50 chromatography, the peptides were in the last peak and were found to be active against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The peptides were conjugated to ovalbumin in order to develop antibodies, which were further tested by Ouchterlony double immunodiffusion and enzyme linked immunosorbent assay. These tests demonstrated the antigenic relationship between buffalo and goats for the PMN cationic peptides as well as the peptide release which was also monitored in activated buffalo PMN leukocytes.
由于缺乏关于中性粒细胞颗粒阳离子蛋白以评估水牛免疫状态的研究，对外周血中性粒细胞进行超声处理和离心循环均质化，以获得纯净的颗粒沉淀。酸提取的颗粒含有阳离子蛋白和肽，通过葡聚糖凝胶G-50排阻色谱进一步分离，肽在最后一个峰中，发现其对革兰氏阴性菌（大肠杆菌）和革兰氏阳性菌（金黄色葡萄球菌）有活性。将这些肽与卵清蛋白偶联以制备抗体，通过双向免疫扩散和酶联免疫吸附测定进一步检测。这些试验证明了水牛和山羊中性粒细胞阳离子肽之间的抗原关系，以及在活化的水牛中性粒细胞中监测到的肽释放情况。
