Fiori P L, Rappelli P, Rocchigiani A M, Cappuccinelli P
Institute of Microbiology and Virology, University of Sassari, Italy.
FEMS Microbiol Lett. 1993 May 1;109(1):13-8. doi: 10.1111/j.1574-6968.1993.tb06136.x.
We have investigated the mechanisms used by Trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. Haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mM EGTA. Osmotic protection experiments using carbohydrates with different molecular diameters as protectants demonstrated that the cytolytic activity of T. vaginalis is inhibited in 75 mM stachyose. On the basis of our data, we hypothesize a cytopathic mechanism mediated by the formation of functional pores into the target membrane. Some of the Trichomonas protein involved in haemolysis have been immunologically characterized.
我们以人类红细胞作为靶细胞，研究了阴道毛滴虫破坏细胞膜的机制。溶血是一种依赖接触和温度的现象，在4 mM乙二醇双四乙酸（EGTA）中会受到抑制。使用不同分子直径的碳水化合物作为保护剂进行的渗透保护实验表明，在75 mM水苏糖中阴道毛滴虫的细胞溶解活性会受到抑制。基于我们的数据，我们推测了一种由在靶膜上形成功能性孔介导的细胞病变机制。一些参与溶血的滴虫蛋白已通过免疫学方法进行了表征。
