Ding W G, Tooyama I, Kimura H, Kuriyama K, Ochi J
Department of Surgery, Shiga University of Medical Science, Otsu, Japan.
Histochem J. 1993 May;25(5):376-83. doi: 10.1007/BF00159502.
The ontogenic pattern of development of taurine-like immunoreactivity (TLI) was studied in the mouse liver. The effect on adult mice of carbon tetrachloride or phenobarbital treatment was also examined. Light-microscopically, granules of TLI were first found in the liver from 17-day-old embryos, diffusely distributed throughout the lobules. These positive granules increased with age, were most numerous in the two-week-old mouse, and were notably decreased in the central region of some lobules in the three-week-old mouse. In mature mice, hepatocytes containing TLI-positive granules were distributed unevenly in each liver lobule, and were located predominantly in the peripheral region. Electron-microscopically, TLI was observed in small vesicles in the cytoplasm of hepatocytes and was found mainly in the cisternal lumen of smooth-surfaced endoplasmic reticulum. Some taurine-positive vesicles surrounding the reticulum seemed to associate with the protoplasm. Similar positive vesicles were often located near the bile canaliculi. In carbon tetrachloride-intoxicated mature mice, TLI was no longer limited to the peripheral region of lobules; hepatocytes situated in the central region of lobules also contained intense TLI. In mice injected with a small and repeated dose of phenobarbital, the distribution pattern of TLI was similar to that in the untreated group. However, in mice injected with a large dose of phenobarbital, TLI was markedly increased, especially in the central region of lobules. The results demonstrate that the distribution pattern of TLI in mouse liver changes during development, and that the pattern in mature mice is affected by intoxication with carbon tetrachloride or a toxic dose of phenobarbital.
研究了小鼠肝脏中牛磺酸样免疫反应性（TLI）的个体发育模式。还检测了四氯化碳或苯巴比妥处理对成年小鼠的影响。光镜下，17日龄胚胎的肝脏中首次发现TLI颗粒，弥漫分布于整个肝小叶。这些阳性颗粒随年龄增加，在两周龄小鼠中最多，在三周龄小鼠的一些肝小叶中央区域明显减少。在成年小鼠中，含有TLI阳性颗粒的肝细胞在每个肝小叶中分布不均，主要位于周边区域。电镜下，TLI见于肝细胞胞质中的小泡内，主要位于滑面内质网的潴泡腔内。一些围绕内质网的牛磺酸阳性小泡似乎与原生质相关。类似的阳性小泡常位于胆小管附近。在四氯化碳中毒的成年小鼠中，TLI不再局限于肝小叶周边区域；位于肝小叶中央区域的肝细胞也含有强烈的TLI。在小剂量反复注射苯巴比妥的小鼠中，TLI的分布模式与未处理组相似。然而，在大剂量注射苯巴比妥的小鼠中，TLI明显增加，尤其是在肝小叶中央区域。结果表明，小鼠肝脏中TLI的分布模式在发育过程中发生变化，成年小鼠中的模式受四氯化碳中毒或有毒剂量苯巴比妥的影响。
