Pollini C P, Giunchedi L, Credi R
Institute of Plant Pathology, University of Bologna, Italy.
J Virol Methods. 1993 Apr;42(1):107-16. doi: 10.1016/0166-0934(93)90182-q.
A dot-immunobinding assay was adapted on enhanced chemiluminescence (DIBA-ECL), which employs luminol, a cyclic diacylhydrazide, as substrate for horseradish peroxidase conjugated with a secondary antibody, for the diagnosis of grapevine closteroviruses I and III. The sensitivity of DIBA-ECL was also compared to other immunoenzymatic methods. DIBA-ECL proved to be at least 16 times more sensitive than the dot-immunobinding assay using chloronaphthol/diaminobenzidine mixture as a substrate, which was at least twice as sensitive as DAS-ELISA, DAS-indirect avidin-biotin complex ELISA, and dot-immunobinding assay, using alkaline phosphatase as enzyme. Optimisation of all parameters involved in the DIBA-ECL procedure and its advantages are discussed.
一种基于增强化学发光的斑点免疫结合测定法（DIBA-ECL）被应用于葡萄藤褪绿斑驳病毒I和III的诊断，该方法使用鲁米诺（一种环状二酰肼）作为与二抗偶联的辣根过氧化物酶的底物。DIBA-ECL的灵敏度也与其他免疫酶法进行了比较。结果表明，DIBA-ECL的灵敏度至少比使用氯萘酚/二氨基联苯胺混合物作为底物的斑点免疫结合测定法高16倍，而后者的灵敏度至少是双抗夹心ELISA、间接抗生物素蛋白-生物素复合物ELISA以及使用碱性磷酸酶作为酶的斑点免疫结合测定法的两倍。本文还讨论了DIBA-ECL程序中所有参数的优化及其优势。
