Sano K, Takasaki T, Fukui A, Jiang Y, Urabe T, Nakano T, Saito Y, Nakamura T, Kise M, Kawabe K
Department of Microbiology, Osaka Medical College.
Kansenshogaku Zasshi. 1993 May;67(5):459-65. doi: 10.11150/kansenshogakuzasshi1970.67.459.
It is important to prevent unexpected laboratory infections in a biosafety area. It is recommended that extreme caution should be used when handling needles and syringes to avoid autoinoculation, and that needles and syringes should be used only when there is no alternative. To remove cells and cell debris, a disk-type microfilter unit, which is recommended using a needle, has been used for filtrating supernatant from HIV-1-infected cell cultures. We compared a stick-type microfilter unit, which can be used without a needle, with a disk-type one for their safety and recovery of human immunodeficiency virus particle after filtration. To confirm the right of our comparison, we observed the filter electron microscopically for the virus particles and virus suspensions were carried out and discussed. In conclusion, the stick-type filter should be improved, since the filter unit is inferior to the disk-type filter unit in purification of the virus, but for the safety is superior to the disk-type.
在生物安全区域预防意外的实验室感染很重要。建议在处理针头和注射器时要格外小心，以避免自我接种，并且只有在没有其他选择时才使用针头和注射器。为了去除细胞和细胞碎片，一种推荐使用针头的盘式微滤装置已被用于过滤来自HIV-1感染细胞培养物的上清液。我们比较了一种无需使用针头即可使用的棒式微滤装置和盘式微滤装置在过滤后人免疫缺陷病毒颗粒的安全性和回收率。为了确认我们比较的正确性，我们通过电子显微镜观察过滤器上的病毒颗粒，并对病毒悬液进行了观察和讨论。总之，棒式过滤器应该改进，因为该过滤装置在病毒纯化方面不如盘式过滤装置，但在安全性上优于盘式。
