Toda M, Tajima N, Ishikawa E, Hashida S
Department of Orthopedic Surgery, Miyazaki Medical College, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1993 Apr;67(4):230-9.
This study was undertaken to investigate the morphological action of chymopapain (CP) in intervertebral discs. Of 20 mature Japanese white rabbits, 19 lumbar intervertebral discs were used for electron microscopic examination, and 36 discs, for immunohistochemical examination. Discs from 3 hours to 8 weeks after injection were observed by light and electron microscopy using anti-CP rabbit antibody labeled with horseradish peroxidase. Using Kagami's DMF-dehydration, two types of fine fibers were observed in the extracellular matrix of normal nucleus pulposus, in addition to collagen fiber. After injection of CP, the thinner fibers disappeared, while the thicker fibers remained. The injected CP spread from the nucleus pulposus to the annulus fibrosus within several hours and remained as long as 4 weeks. By immunoelectron microscopy, positive granules were divided into Type 1 (lacking relationship to collagen fibers) and Type 2 (adhering to or surrounding collagen fibers). The thinner fibers appear to be proteoglycan monomer and are the target for CP.
本研究旨在探讨木瓜凝乳蛋白酶（CP）在椎间盘的形态学作用。在20只成年日本白兔中，19个腰椎间盘用于电子显微镜检查，36个椎间盘用于免疫组织化学检查。使用辣根过氧化物酶标记的抗CP兔抗体，通过光镜和电镜观察注射后3小时至8周的椎间盘。使用镜见氏DMF脱水法，除胶原纤维外，在正常髓核的细胞外基质中观察到两种类型的细纤维。注射CP后，较细的纤维消失，而较粗的纤维保留。注射的CP在数小时内从髓核扩散到纤维环，并持续长达4周。通过免疫电子显微镜观察，阳性颗粒分为1型（与胶原纤维无关联）和2型（附着于或围绕胶原纤维）。较细的纤维似乎是蛋白聚糖单体，是CP的作用靶点。
