Kitano S, Tsuji H, Hirano N, Sano A, Terahata N
Department of Orthopaedic Surgery, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Spine (Phila Pa 1976). 1989 Nov;14(11):1226-33. doi: 10.1097/00007632-198911000-00016.
Chymopapain (Discase) was injected at a dose of 0.125 nanokatal unit into the intervertebral discs of rabbits, and sequential changes in the metabolism of water, proteoglycan, collagen, and noncollagenous protein were investigated separately in the nucleus pulposus, anterior, and posterior anulus fibrosus. One week after chymopapain injection, the water and proteoglycan content was lower in all of the fractionated tissues of the anterior and posterior anulus and nucleus pulposus of the discs than in the control discs. In the anterior and posterior anulus, the proteoglycan content recovered after 12 weeks, but there was no recovery in the nucleus pulposus. The collagen content continued to increase up to the 12th week in the nucleus pulposus, while the noncollagenous protein content decreased in all tissue fractions after 1 week. In the anterior and posterior anulus, the content of noncollagenous protein recovered after 3 to 6 weeks, but there was no recovery in the nucleus pulposus. The lysine incorporation in collagen and noncollagenous protein was inhibited in all tissue fractions after 12 weeks, suggesting a decrease in synthetic activity. The intradiscal pressure calculated from proteoglycan hydration at 1 to 6 weeks after chymopapain injection showed a marked decrease to 0.8 to 0.9 atm, but it recovered to 1.6 atm after 12 weeks.
将木瓜凝乳蛋白酶（椎间盘溶解酶）以0.125纳 katal单位的剂量注射到兔椎间盘内，分别研究髓核、纤维环前部和后部中水、蛋白聚糖、胶原蛋白和非胶原蛋白代谢的顺序变化。注射木瓜凝乳蛋白酶一周后，椎间盘纤维环前部和后部以及髓核的所有分级组织中的水和蛋白聚糖含量均低于对照椎间盘。在纤维环前部和后部，12周后蛋白聚糖含量恢复，但髓核中未恢复。髓核中的胶原蛋白含量在第12周前持续增加，而所有组织分级中的非胶原蛋白含量在1周后下降。在纤维环前部和后部，3至6周后非胶原蛋白含量恢复，但髓核中未恢复。12周后，所有组织分级中胶原蛋白和非胶原蛋白中的赖氨酸掺入均受到抑制，表明合成活性降低。木瓜凝乳蛋白酶注射后1至6周根据蛋白聚糖水合作用计算的椎间盘内压显著下降至0.8至0.9个大气压，但12周后恢复至1.6个大气压。
