Zhang M, Hausman D B, Hausman G J
Department of Foods and Nutrition, University of Georgia, Athens 30602.
J Nutr. 1993 Jul;123(7):1203-7. doi: 10.1093/jn/123.7.1203.
This study examined the influence of dietary essential fatty acids on the cooperativity of isolated adipocytes and stromal-vascular cells in the biosynthesis of prostaglandins. Sprague-Dawley rats were fed either a diet rich in essential fatty acids (20% corn oil) or a diet poor in essential fatty acids (20% tallow) for 4 wk. Preparations containing primarily adipose cells (adipocytes) or stromal-vascular cells (nonfat cells) were obtained from epididymal fat pads by collagenase digestion and repeatedly washed. Prostaglandin production was evaluated in basal and epinephrine-stimulated media after incubation with either adipocytes or adipocytes+nonfat cells. Prostaglandin E2 and prostacyclin production by adipocytes+nonfat cells was dose-dependent with epinephrine stimulation in cells from rats fed both diets. Both prostaglandin E2 and glycerol release in response to epinephrine (10-100 mumol/L) stimulation from adipocytes or from adipocytes+nonfat cells were significantly higher for cells from corn oil-fed rats than for cells from tallow-fed rats. Regardless of dietary treatment, epinephrine-stimulated prostaglandin E2 and prostacyclin release from adipocytes+nonfat cells was much greater than from adipocytes. These results suggest that a diet high in essential fatty acids precipitates a higher prostaglandin E2 secretion and that nonfat cells potentiate the secretion of prostaglandin E2 and prostacyclin by adipocytes regardless of diet.
本研究考察了膳食必需脂肪酸对分离的脂肪细胞和基质血管细胞在前列腺素生物合成中的协同作用的影响。将斯普拉格-道利大鼠分别喂食富含必需脂肪酸的饮食（20%玉米油）或缺乏必需脂肪酸的饮食（20%牛脂）4周。通过胶原酶消化从附睾脂肪垫中获得主要含有脂肪细胞（脂肪细胞）或基质血管细胞（非脂肪细胞）的制剂，并反复洗涤。在与脂肪细胞或脂肪细胞+非脂肪细胞孵育后，在基础培养基和肾上腺素刺激的培养基中评估前列腺素的产生。在两种饮食喂养的大鼠的细胞中，脂肪细胞+非脂肪细胞产生的前列腺素E2和前列环素与肾上腺素刺激呈剂量依赖性。来自玉米油喂养大鼠的细胞中，脂肪细胞或脂肪细胞+非脂肪细胞对肾上腺素（10 - 100 μmol/L）刺激的前列腺素E2和甘油释放均显著高于来自牛脂喂养大鼠的细胞。无论饮食处理如何，肾上腺素刺激下脂肪细胞+非脂肪细胞释放的前列腺素E2和前列环素均远高于脂肪细胞。这些结果表明，富含必需脂肪酸的饮食会促使更高的前列腺素E2分泌，并且无论饮食如何，非脂肪细胞都会增强脂肪细胞分泌前列腺素E2和前列环素的能力。
