Sanada T, Nakamura T, Nishimura M C, Isayama K, Pitts L H
Department of Neurological Surgery, School of Medicine, University of California, San Francisco.
J Neurotrauma. 1993 Spring;10(1):65-71. doi: 10.1089/neu.1993.10.65.
The effect of the 21-aminosteroid U74006F, an inhibitor of iron-dependent lipid peroxidation, on neurologic outcome and cerebral edema was evaluated in adult male Sprague-Dawley rats subjected to a fluid percussion temporal brain injury followed by 45 min of hypoxia (PaO2 = 30.0 mm Hg). The rats were divided randomly into five groups. Bolus injections of a control drug or U74006F (1.0, 3.0, 10.0, or 30.0 mg/kg) were given 3 min and 3 h after the injury. Twenty-four hours after the injury, the neurologic status was evaluated, the rats were killed, and brain water content was determined by microgravimetry. U74006F did not significantly reduce brain water content at any dose level, nor did it affect rotorod walking or activity scores. However, rats treated with U74006F at a dose of 10.0 mg/kg had significantly better motor function scores (p < 0.05) than rats in the control group. These findings demonstrate the usefulness of U74006F as a cerebroprotective agent in this model of experimental head injury.
在成年雄性斯普拉格-道利大鼠中，评估了铁依赖性脂质过氧化抑制剂21-氨基类固醇U74006F对神经功能结局和脑水肿的影响，这些大鼠先经历流体冲击性颞脑损伤，随后进行45分钟的缺氧（动脉血氧分压=30.0毫米汞柱）。将大鼠随机分为五组。在损伤后3分钟和3小时给予对照药物或U74006F（1.0、3.0、10.0或30.0毫克/千克）的大剂量注射。损伤后24小时，评估神经状态，处死大鼠，并通过微量重力测定法测定脑含水量。U74006F在任何剂量水平下均未显著降低脑含水量，也未影响转棒行走或活动评分。然而，用10.0毫克/千克剂量的U74006F治疗的大鼠的运动功能评分明显优于对照组大鼠（p<0.05）。这些发现证明了U74006F在这种实验性头部损伤模型中作为脑保护剂的有效性。
