Edward Z, Takegami T
Division of Tropical Medicine, Kanazawa Medical University, Ishikawa, Japan.
Microbiol Immunol. 1993;37(3):239-43. doi: 10.1111/j.1348-0421.1993.tb03206.x.
Recently it has been reported that Japanese encephalitis virus (JEV)-specific RNAs can be synthesized in vitro in the subcellular fraction including outer-nuclear membrane (Takegami and Hotta, 1989). The results of Western blot analysis and indirect immunofluorescence test using two kinds of monospecific antisera against JEV nonstructural proteins NS3 and NS5 showed that NS3 and NS5 were membrane-associated proteins and formed the complex at the perinuclear site in the infected cells. Both antisera against NS3 and NS5 inhibited in vitro RNA synthesis. These results suggest that NS5 and NS3 play important role(s) in flavivirus RNA replication.
最近有报道称，日本脑炎病毒（JEV）特异性RNA可在包括外核膜的亚细胞组分中体外合成（武上和堀田，1989年）。使用两种针对JEV非结构蛋白NS3和NS5的单特异性抗血清进行的蛋白质免疫印迹分析和间接免疫荧光试验结果表明，NS3和NS5是膜相关蛋白，并在受感染细胞的核周部位形成复合物。针对NS3和NS5的两种抗血清均抑制体外RNA合成。这些结果表明，NS5和NS3在黄病毒RNA复制中起重要作用。
