Mooradian A D, Smith T L
St. Louis Department of Veterans Affairs Medical Center, MO.
Neurobiol Aging. 1993 May-Jun;14(3):229-32. doi: 10.1016/0197-4580(93)90005-v.
To determine if the membrane disordering effect of ethanol on cerebral microvessels is altered with age, the steady state fluorescence polarization (P) of diphenyl-hexatriene (DPH) and 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) which report on the membrane core and surface region, respectively, was determined in isolated cerebral microvessel membranes from 3-month-old (young) and 24-month-old (aged) male Fischer 344 rats in the absence or presence of 0, 25, 50, 100, 200, and 400 mM ethanol. The addition of ethanol resulted in concentration dependent decrease in DPH fluorescence polarization in both young and aged rat cerebral microvessels. Analysis of variance indicated that these two age groups did not differ from each other in their response to ethanol over this concentration range or in their baseline P. However, the TMA-DPH fluorescence polarization in cerebral microvessels in the absence or presence of various concentrations of ethanol was significantly lower in aged rats (p < 0.01). It is concluded that aging in rats is associated with a decreased membrane lipid order near the membrane surface of cerebral microvessels as assessed by TMA-DPH. Membrane disordering effect of ethanol on cerebral microvessels does not appear to be altered significantly with age.
为了确定乙醇对脑微血管的膜紊乱作用是否随年龄而改变，分别在不存在或存在0、25、50、100、200和400 mM乙醇的情况下，测定了来自3个月大（年轻）和24个月大（老年）雄性Fischer 344大鼠的离体脑微血管膜中分别报告膜核心和表面区域的二苯基己三烯（DPH）和1-（4-三甲基铵苯基）-6-苯基-1,3,5-己三烯（TMA-DPH）的稳态荧光偏振（P）。乙醇的添加导致年轻和老年大鼠脑微血管中DPH荧光偏振呈浓度依赖性降低。方差分析表明，在该浓度范围内，这两个年龄组对乙醇的反应或其基线P彼此无差异。然而，在不存在或存在各种浓度乙醇的情况下，老年大鼠脑微血管中的TMA-DPH荧光偏振显著较低（p < 0.01）。结论是，通过TMA-DPH评估，大鼠衰老与脑微血管膜表面附近的膜脂有序性降低有关。乙醇对脑微血管的膜紊乱作用似乎不会随年龄而显著改变。
