Hildreth S W, Baggs R R, Brownstein D G, Castleman W L, Paradiso P R
Virology Research, Praxis Biologics, Rochester, NY 14623.
Vaccine. 1993;11(6):615-8. doi: 10.1016/0264-410x(93)90304-g.
The cotton rat model has been used to evaluate the potential for immunogens to induce respiratory syncytial virus (RSV)-enhanced pulmonary histopathology. A recent study evaluated purified F protein in this model when animals were challenged intranasally with RSV 3 or 6 months after immunization. The authors concluded that the purified F protein was associated with the same level of histopathological changes as observed with the positive control, a formalin-inactivated RSV immunogen. Three pathologists have independently evaluated the lung sections from the animals of this study and the results are reported in this article. In contrast to the previously published data, we have found that F protein was associated with a substantially milder and qualitatively different response to that observed with the formalin-inactivated RSV vaccine. We concluded that the minimal histological changes observed and lack of clinical disease make it very difficult to assess the issue of enhanced pulmonary RSV disease with the cotton rat model.
棉鼠模型已被用于评估免疫原诱导呼吸道合胞病毒（RSV）增强型肺部组织病理学的可能性。最近一项研究在该模型中评估了纯化的F蛋白，免疫动物在免疫后3或6个月经鼻用RSV攻击。作者得出结论，纯化的F蛋白与阳性对照（福尔马林灭活的RSV免疫原）所观察到的组织病理学变化水平相同。三位病理学家独立评估了本研究动物的肺切片，结果报告于本文。与先前发表的数据相反，我们发现F蛋白所引发的反应比福尔马林灭活的RSV疫苗所观察到的反应明显更轻且性质不同。我们得出结论，观察到的最小组织学变化以及缺乏临床疾病使得用棉鼠模型评估RSV增强型肺部疾病问题非常困难。
