Ballesteros I, Oliva J M, Ballesteros M, Carrasco J
Instituto de Energias Renovables, CIEMAT Avda, Madrid, Spain.
Appl Biochem Biotechnol. 1993 Spring;39-40:201-11. doi: 10.1007/BF02918990.
Different treatments to improve the thermotolerance of fermenting yeasts for simultaneous ethanol saccharification and fermentation process of cellulosic materials have been examined. Yeasts of the genera Saccharomyces and Kluyveromyces were tested for growth and fermentation at progressively higher temperatures in the range of 42-47 degrees C. The best results were obtained with K. marxianus LG, which was then submitted to different treatments in order to achieve thermotolerant clones. A total of 35 new clones were obtained that dramatically improved the SSF of 10% Solka-floc substrate at 45 degrees C when compared to the original strain, some with ethanol concentrations as high as 33 g/L.
已对不同处理方法进行研究，以提高发酵酵母的耐热性，用于纤维素材料的同步乙醇糖化和发酵过程。对酿酒酵母属和克鲁维酵母属的酵母在42-47摄氏度范围内逐渐升高的温度下进行生长和发酵测试。用马克斯克鲁维酵母LG获得了最佳结果，然后对其进行不同处理以获得耐热克隆。总共获得了35个新克隆，与原始菌株相比，这些克隆在45摄氏度下显著提高了10% Solka-floc底物的同步糖化发酵效果，有些克隆的乙醇浓度高达33 g/L。
