Hari Krishna S, Janardhan Reddy T, Chowdary G V
Biotechnology Division, Department of Chemical Engineering, Andhra University, Visakhapatnam 530 003, India.
Bioresour Technol. 2001 Apr;77(2):193-6. doi: 10.1016/s0960-8524(00)00151-6.
Simultaneous saccharification and fermentation (SSF) studies were carried out to produce ethanol from lignocellulosic wastes (sugar cane leaves and Antigonum leptopus leaves) using Trichoderma reesei cellulase and yeast cells. The ability of a thermotolerant yeast, Kluyveromyces fragilis NCIM 3358, was compared with Saccharomyces cerevisiae NRRL-Y-132. K. fragilis was found to perform better in the SSF process and result in high yields of ethanol (2.5-3.5% w/v) compared to S. cerevisiae (2.0-2.5% w/v). Increased ethanol yields were obtained when the cellulase was supplemented with beta-glucosidase. The conversions with K. fragilis were completed in a short time. The substrates were in the following order in terms of fast conversions: Solka floc > A. leptopus > sugar cane.
进行了同步糖化发酵（SSF）研究，以利用里氏木霉纤维素酶和酵母细胞从木质纤维素废料（甘蔗叶和戟叶蓼叶）中生产乙醇。将耐热酵母脆壁克鲁维酵母NCIM 3358的能力与酿酒酵母NRRL-Y-132进行了比较。结果发现，与酿酒酵母（2.0-2.5% w/v）相比，脆壁克鲁维酵母在SSF过程中表现更好，乙醇产量更高（2.5-3.5% w/v）。当纤维素酶中添加β-葡萄糖苷酶时，乙醇产量增加。脆壁克鲁维酵母的转化在短时间内完成。就快速转化而言，底物的顺序如下：索尔卡纤维>戟叶蓼>甘蔗。
